rabbit polyclonal anti rap1  (Novus Biologicals)

Journal: Scientific Reports

Article Title: CD147 regulates the Rap1 signaling pathway to promote proliferation, migration, and invasion, and inhibit apoptosis in colorectal cancer cells

doi: 10.1038/s41598-025-98266-8

Figure Lengend Snippet: Information of primary antibodies used in the present study.

Article Snippet: Anti RAP1 rabbit polyclonal antibody , 14595-1-AP , ProteinTech Group, Inc. , 1:1000.

Techniques:

Journal: Scientific Reports

Article Title: CD147 regulates the Rap1 signaling pathway to promote proliferation, migration, and invasion, and inhibit apoptosis in colorectal cancer cells

doi: 10.1038/s41598-025-98266-8

Figure Lengend Snippet: Primer sequence and promoter primer sequence.

Article Snippet: Anti RAP1 rabbit polyclonal antibody , 14595-1-AP , ProteinTech Group, Inc. , 1:1000.

Techniques: Sequencing

Journal: Scientific Reports

Article Title: CD147 regulates the Rap1 signaling pathway to promote proliferation, migration, and invasion, and inhibit apoptosis in colorectal cancer cells

doi: 10.1038/s41598-025-98266-8

Figure Lengend Snippet: CD147 overexpression correlates with poor prognosis and RAP1 co-expression in colorectal cancer. ( A , B ) CD147 mRNA expression levels in tumor vs. normal tissues across multiple cancers (UCSC Xena database) and specifically in colorectal cancer (CRC) (GEPIA database). ** P < 0.01, unpaired t-test. ( C ) Kaplan-Meier survival analysis of CRC patients stratified by CD147 expression (log-rank test, P < 0.05). ( D ) Representative immunohistochemical images from The Human Protein Atlas showing CD147 expression in normal colorectal tissue (negative) and CRC tissue (cytoplasmic/membrane staining). ( E , F ) Western blot analysis of CD147 protein levels in paired CRC and adjacent normal tissues ( n = 12). β-actin served as loading control. *** P < 0.001, paired t-test. Origin blots are presented in Supplementary Fig. 1. ( G , H ) Correlation analyses between CD147 and RAP1A/B mRNA expression using GEPIA (Pearson’s correlation, P < 0.05) and TIMER2.0 (no significant correlation). ( I ) qRT-PCR validation of CD147 and Rap1 expression correlation in 12 CRC tissues (Pearson’s correlation, P < 0.01).

Article Snippet: Anti RAP1 rabbit polyclonal antibody , 14595-1-AP , ProteinTech Group, Inc. , 1:1000.

Techniques: Over Expression, Expressing, Immunohistochemical staining, Membrane, Staining, Western Blot, Control, Quantitative RT-PCR, Biomarker Discovery

Journal: Scientific Reports

Article Title: CD147 regulates the Rap1 signaling pathway to promote proliferation, migration, and invasion, and inhibit apoptosis in colorectal cancer cells

doi: 10.1038/s41598-025-98266-8

Figure Lengend Snippet: Rap1 overexpression reverses the expression of key proteins in CD147 knockdown mediated cell proliferation, apoptosis and EMT. ( A , B ) Western blot analysis of Rap1 and Rap1GAP protein levels in HCT116 and SW620 cells after CD147 knockdown (shCD147). β-actin served as a loading control. Data represent mean ± SD ( n = 3; ** P < 0.01, *** P < 0.001 vs. shNC, Student’s t-test). Original blots are presented in Supplementary Fig. 3. ( C ) qRT-PCR validation of Rap1 mRNA overexpression in CD147-knockdown cells. Expression normalized to β-actin ( n = 3; *** P < 0.001 vs. HCT116 or SW620, Student’s t-test). ( D , E ) Western blot analysis of c-Myc, Bcl-2, Bax, N-cadherin, and E-cadherin protein levels in CD147-Knockdown cells with Rap1 overexpression ( n = 3; * P < 0.05, ** P < 0.01, *** P < 0.001 vs. shNC, # P < 0.05, ## P < 0.01, ### P < 0.001 vs. shCD147, one-way ANOVA). Original blots are presented in Supplementary Fig. 4.

Article Snippet: Anti RAP1 rabbit polyclonal antibody , 14595-1-AP , ProteinTech Group, Inc. , 1:1000.

Techniques: Over Expression, Expressing, Knockdown, Western Blot, Control, Quantitative RT-PCR, Biomarker Discovery

Journal: Scientific Reports

Article Title: CD147 regulates the Rap1 signaling pathway to promote proliferation, migration, and invasion, and inhibit apoptosis in colorectal cancer cells

doi: 10.1038/s41598-025-98266-8

Figure Lengend Snippet: Rap1 overexpression rescues the regulatory effects of CD147 knockdown on proliferation and apoptosis. ( A ) CCK-8 assay showing proliferation of HCT116 ( n = 3; *** P < 0.001 vs. shNC, ### P < 0.001 vs. shCD147, one-way ANOVA). ( B , C ) Colony formation assays in HCT116 cells. Colonies were counted and normalized to shCtrl ( n = 3; ** P < 0.01, *** P < 0.001 vs. shNC, ### P < 0.001 vs. shCD147, one-way ANOVA). ( D , E ) Flow cytometry analysis of apoptosis in HCT116 cells. Percentages of apoptotic cells are shown ( n = 3; * P < 0.05 vs. shNC, # P < 0.05 vs. shCD147, one-way ANOVA). Similar results were observed in SW620 cells.

Article Snippet: Anti RAP1 rabbit polyclonal antibody , 14595-1-AP , ProteinTech Group, Inc. , 1:1000.

Techniques: Over Expression, Knockdown, CCK-8 Assay, Flow Cytometry

Journal: Scientific Reports

Article Title: CD147 regulates the Rap1 signaling pathway to promote proliferation, migration, and invasion, and inhibit apoptosis in colorectal cancer cells

doi: 10.1038/s41598-025-98266-8

Figure Lengend Snippet: Rap1 restores CD147-mediated migration and invasion in colorectal cancer cells. ( A , C ) Scratch wound healing assay in HCT116 cells. Healing rates were quantified at 24 h and 48 h ( n = 3; *** P < 0.001 vs. shNC, ## P < 0.01, ### P < 0.001 vs. shCD147, one-way ANOVA). ( B , D ) Transwell migration and Matrigel invasion assays. Migrated/invaded cells were counted and normalized to shCtrl ( n = 3; *** P < 0.001 vs. shNC, ### P < 0.001 vs. shCD147, one-way ANOVA). SW620 cells showed consistent trends. ( E ) Mechanistic diagram of CD147 promoting tumor progression through Rap1/Rap1GAP signaling in colorectal cancer cells.

Article Snippet: Anti RAP1 rabbit polyclonal antibody , 14595-1-AP , ProteinTech Group, Inc. , 1:1000.

Techniques: Migration, Wound Healing Assay

Journal: Journal of immunology (Baltimore, Md. : 1950)

Article Title: Crosstalk between Prostaglandin E 2 and Leukotriene B 4 Regulates Phagocytosis in Alveolar Macrophages Via Combinatorial Effects on Cyclic AMP

doi: 10.4049/jimmunol.182.1.530

Figure Lengend Snippet: Counterregulation of cAMP effectors by PGE2 and LTs. (A). AMs were preincubated with PGE2 (100 nM), LTB4 (10 nM), LTD4 (100 nM), PGE2 plus LTB4, PGE2 plus LTD4 or vehicle control for 15 min, lysed, and assayed for GTP-bound Rap1 as described in “Materials and Methods”. Densitometric analysis was performed for each western blot as described in “Materials and Methods.” (B). AMs were stimulated with PGE2 (100 nM), LTB4 (10 nM), LTD4,(100 nM), PGE2 plus LTB4, PGE2 plus LTD4 or vehicle control for 15 min, after which the proteins were serparated by SDS-PAGE and then the membranes were probed for pVASP (1:500). Subsequently, the membrane was stripped and probed for β-actin (1:1,000). Relative phosphorylation was determined by densitometric analysis of immunoblots from three different experiments and expressed as percentage of control. *, p<0.001 versus control; §, p<0.001 versus PGE2.

Article Snippet: Samples were submitted to SDS-PAGE and the membranes were probed with polyclonal rabbit anti-Rap1 antibody (1:500; Upstate Biotechnology) and bands detected as described in the following section.

Techniques: Control, Western Blot, SDS Page, Membrane, Phospho-proteomics

Journal: Breast Cancer Research : BCR

Article Title: Extracellular calcium increases bisphosphonate-induced growth inhibition of breast cancer cells

doi: 10.1186/bcr1845

Figure Lengend Snippet: Effect of calcium on ibandronate-induced inhibition of Rap1A prenylation in MDA-MB-231 and MCF-7 cells. Cells were incubated for 24 hours with increasing concentrations of ibandronate (1 to 1,000 μmol/l) in culture medium containing 0.6 or 2.0 mmol/l calcium, lysed and subjected to Western blot analysis. Equal amounts of proteins (25 μg) were subjected to 12% SDS-PAGE and electrotransferred onto nitrocellulose membranes. Immunodetection was performed with a goat polyclonal anti-human antibody raised against the unprenylated form of Rap1A. Total Rap1 protein (input) was evaluated in parallel using a rabbit polyclonal anti-human Rap1 antibody. Representative data from two separate experiments.

Article Snippet: Equal amounts of protein were subjected to Western blotting using a goat polyclonal anti-human Rap1A antibody (SC-1482; Santa Cruz Biotechnology, Santa Cruz, CA, USA) diluted 1:1,000, which recognizes the unprenylated form of the small GTPase Rap1A [ ], and a rabbit polyclonal anti-human Rap1 antibody (SC-65; Santa Cruz Biotechnology) diluted 1:500.

Techniques: Inhibition, Incubation, Western Blot, SDS Page, Immunodetection